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KMID : 0378019810240020113
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New Medical Journal
1981 Volume.24 No. 2 p.113 ~ p.117
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Articular Innervations and their Pathways studied with Horseradish Peroxidase
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Abstract
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The previous works for the innervation of the articular capsule referred mostly to the morphophysiological dispositions of the nerve fibers and their endings, but little to their pathways to the central nervous system.
This experiment was undertaken in an attempt to elucidate that problem taking an advantage of the retrograde intraaxonal transport of horseradish peroxidase(HRP).
A group of animals, under anesthesis, was intubated a polyethylene tube into the joint cavity of each, and through it, HRP was infused followed by a infusion of a corrosive (2% silver nitrate) solution to destruct the synovial layer.
Another group was exposed and opened the articular sac, and HRP was variously injected into the capsular tissue by a microsyringe under stereomicroscope.
After fifty to sixty hours of survival, the animals were fixed by perfusion method and removed sympathetic and spinal ganglia and spinal cord. Serial frozen sections were made and processed for demonstration of HRP by method adapted from Graham and Karnovsky.
The conclusions obtained from this investigation are summarized as followings.
1. It was affirmed that the articular capsule was innervated by the sympathetic and the somatic sensory nerves.
2. The sympathetic fibers were arised from the sixth and seventh lumbar and the first and second sacral ganglia, but the sensory fibers mostly from the seventh lumbar and the first sacral and a few from the second sacral.
3. The HRP diffused into the articular capsule was considered to be removed largely by the absorption of the blood capillaries.
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